description
specification
resources
service description
bgi genomics has extensive experience in the field of untargeted metabolomics with developed reliable workflows using market leading technologies and an advanced bioinformatics infrastructure.
untargeted metabolomics
by applying cutting-edge lc-ms/ms systems and techniques, bgi genomics offers validated workflows of untargeted metabolomics to support biomedical and biotechnology applications¹. our untargeted metabolomics services are designed to obtain a metabolite profile and screen for differentially-expressed molecules in the sample.
project workflow
references
[1] liu r., et al., gut microbiome and serum metabolome alterations in obesity and after weight-loss intervention. nat med, 2017. 23(7): p. 859-868.
how to order
mass spectrometry service specification
untargeted metabolomics services are performed using waters acquity uplc and cutting-edge mass spectrometer (q exactive hf/hf-x and q-tof 5600).
sample preparation and services
- increase metabolome depth of coverage using both acquity uplc beh c18 and amide column
- ( ) and (-) esi detection by a q exactive hf/hf-x mass spectrometer
quality standard
- summary includes all methods and data analysis
- reports provided in excel or pdf format, raw files available upon request
turn around time
- typical 5-7 weeks after sample receipt for raw data delivery
sample requirements
we accept metabolite samples in a variety of formats. contact your bgi genomics account representative for details for your samples.
| sample type | recommended sample amount | minimum sample amount |
|---|---|---|
| serum, plasma | ≥ 300 µl | ≥ 100 µl |
| urine | ≥ 300 µl | ≥ 100 µl |
| animal and clinical tissues | ≥ 200 mg | ≥ 25 mg |
| feces and intestinal contents | ≥ 200 mg | ≥ 25 mg |
| cell | 1×10^7 | 5×10^6 |
| microorganism | ≥ 200 mg | ≥ 25 mg |
| culture medium, fermentation medium | ≥ 1 ml | ≥ 100 µl |
| milk | ≥ 1 ml | ≥ 100 µl |
| other body fluids (amniotic fluid, saliva, hemolymph, cerebrospinal fluid, etc.) | ≥ 300 µl | ≥ 100 µl |
data analysis
- data analysis performed with metax1 and bgi genomics library
- t-test, pca, pls-da, opls-da for differential metabolite identification
- cluster analysis
- metabolite pathway annotation
- roc analysis
- metabolite correlation analysis