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rna sequencing (transcriptome)
our world leading rna sequencing services are relied upon by researchers and companies all over the world. our scale and experience enable us to offer industry beating pricing, highly efficient turnaround times and fantastic project support. contact us today with any questions or for a no obligation quote.
rna sequencing introduction
rna sequencing, sometimes referred to as transcriptome sequencing, is used to reveal the presence, quantity and structure of rna in a biological sample under specific conditions. compared to hybridization-based rna quantification methods such as microarray analysis, sequencing-based transcriptome detection can quantify gene expression with low background, high accuracy and high levels of reproducibility within a large dynamic range. in addition, transcriptome sequencing does not require an existing genome sequence and can detect splice variants and fusion genes that can not be detected by microarrays.
with over two decades of experience, we have supported most of the world’s leading research institutes and pharma companies as well as many thousands of other partners across the globe. our experience, sequencing scale and proprietary technology enables quality rna data, rapidly delivered, all at industry beating pricing. contact us today for a no obligation quote.
over 20 years of experience with millions of rna samples processed.
guaranteed data quality at competitive pricing enabled by proprietary technology.
rapid turnaround times and expert support, no project too big or small.
rna sequencing (transcriptome)-bgi service overview
sample requirements
human/mice/rat (non-whole blood)
total rna: ≥ 200ng,
concentration: ≥ 10ng/μl
general quality requirements:
rin: ≥ 7.0
28s/18s: ≥ 1.0
(note, we can process degraded samples at lower quality thresholds. contact us for more information.)
sequencing standards
• guaranteed ≥ 80% of bases with quality score of ≥ q30
• 100bp and 150bp paired-end sequencing options available
• ≥30 million reads per sample recommended
key service details
• typical 25 working days, fast 18 working days from sample qc acceptance to filtered raw data availability
• expedited services are available, contact us for more detail
• reports and output data files are delivered in industry standard file formats: bam, .xls, .png and fastq data.
unique dnbseq™ sequencing technology
bgi's rna sequencing services are typically executed with proprietary dnbseq™ sequencing technology platforms, for great sequencing data at some of the lowest costs in the industry. dnbseq™ offers advantages in terms of lower amplification error rates and much lower duplication rates. in addition, studies have shown the lower index hopping rate in dnbseq™ platforms.
contact us with any general enquiries or questions for our sequencing experts, we are here to help.
access further sequencing resources
we care for your samples from the start through to the result reporting. highly experienced laboratory professionals follow strict quality procedures to ensure the integrity of your results. access more sequencing resources below for full access to our range of educational resources and sample guides.
register for a bgi account
opening a bgi customer account enables you to request a quote and order services from us. it also provides access to our range of educational resources including educational webinars providing practical sequencing tips and our complete sample preparation guides.