ptm proteomics -凯发天生赢家一触即发官网

description

specification

resources

service description

we have developed innovative solutions for optimizing the processes of ptm identification of purified protein, modified protein profiling, and protein ptm quantification in complex biological matrices and other complex mixtures.

ptm proteomics

post-translational modification (ptm) of proteins refers to the chemical changes proteins may undergo after translation. common ptms include phosphorylation, glycosylation, acetylation and ubiquitination. as a result, identifying and understanding ptms is key for the study of cell biology and the treatment and prevention of disease.

bgi genomics has extensive experience in the field of ptm proteomics1,2 and has developed reliable workflows using market leading technologies and an advanced bioinformatics infrastructure.

project workflow

phosphorylation:

acetylation/ubiquitylation:

protein extraction
protein digestion
modified peptides enrichment
hplc fractionation
lc-ms/ms (dda)
data analysis

glycosylaion:

quantification of n-glycosylated peptides

protein extraction
protein digestion
modified peptides enrichment
remove glycan chains
lc-ms/ms(dda)
data analysis

quantification of n-intact glycopeptides

protein extraction
protein digestion
isobaric labeling and enrichment
hplc fractionation
lc-ms/ms
analysis by strucgp

references

[1] zhang, y., et al., evidence for differential glycosylation of trophoblast cell types. mol cell proteomics, 2016 jun;15(6):1857-66. doi: 10.1074/mcp.m115.055798.

[2] hao p l, et al., correction of errors in tandem mass spectrum extraction enhances phosphopeptide identification. j. proteome res., 2013, 12, 12, 5548–5557. doi: 10.1021/pr4004486.

how to order

request a quote

mass spectrometry service specification

ptm proteomics services are performed using nano-flow liquid chromatography and cutting-edge mass spectrometer (q exactive hf/hf-x and orbitrap fusion lumos).

sample preparation and services
- digestion performed using sequencing-grade trypsin
- off-line sample fractionation using hplc technology
- each fraction analyzed using nano-flow lc-ms/ms
- tio₂/zro₂ enrichment for phosphoproteomics; cst antibody enrichment for acetylated/ ubiquitylated proteins; max column enrichment for glycosylated proteins
quality standard
- summary includes all methods and data analysis
- reports provided in excel or pdf format, raw files available upon request
turn around time
- phosphorylation turn around time from sample qc acceptance to data report delivery 3-5 weeks
- glycosylation turn around time from sample qc acceptance to data report delivery 4-5 weeks
- acetylation/ubiquitylation turn around time from sample qc acceptance to data report delivery 3-5 weeks