description
specification
resources
project workflow
gel spot identification
we can identify proteins present in gel spots.
- gel spot
- decolorization
- protein digestion
- lc-ms/ms (dda)
- protein identification
gel band identification
we can identify proteins present in gel bands or protein solution.
- gel band or protein solution
- decolorization
- protein digestion
- lc-ms/ms (dda)
- bioinformatics analysis
how to order
service specification
sample preparation and services
- digestion performed using sequencing-grade trypsin
- 60 min nano-flow lc-ms/ms
quality standard
- summary includes all methods and data analysis
- reports provided in excel or pdf format, raw files available upon request
turn around time
- typical 2 weeks after sample receipt for raw data delivery
sample requirements
projects that need enrichment for acetylation and ubiquitination studies is not accepted.
| product sample | gel bandidentification | modification site identification |
|---|---|---|
| coomassive or silver stained protein spot/band | protein ≥ 1 μg | protein ≥ 1μg. projects that need enrichment is not accepted. |
| single protein (protein solution purified after ip or co-ip) | ≥ 5 μg,≥ 0.5 μg/μl | ≥ 10 μg, ≥ 0.5 μg/μl. if enrichment is required, the amount of sample sent needs to be increased by 3 times. |
data analysis
advanced bioinformatics
- data analysis and validation performed with mascot
- go (gene ontology) category analysis
- cog (cluster of orthologous groups of proteins) category analysis
- pathway analysis