description
specification
resources
service description
we have developed innovative solutions for optimizing the process of comprehensive qualitative and quantitative analysis of all endogenous peptides in complex biological matrices and other complex mixtures.
peptidomics
peptidomics is an emerging field filling the research gap between proteomics and metabolomics and can be defined as the comprehensive qualitative and quantitative analysis of all endogenous peptides from biological samples at a specific time and location. bgi genomics has extensive experience in the field of peptidomics spanning disease biomarker study, off target effects of drugs, food processing and fermentation research, the molecular mechanisms of animals and plants, and cell antigen epitopes prediction1,2.
project workflow
- biological samples
- enrichment of endogenous polypeptides
- lc-ms/ms analysis
- data analysis
references
[1] chen x y, et al., high-throughput identification of putative antimicrobial peptides from multi-omics data of the lined seahorse (hippocampus erectus). mar. drugs, 2020, 18(1), 30. doi: 10.3390/md18010030.
[2] yi y h, et al., high throughput identification of antihypertensive peptides from fish proteome datasets. mar drugs, 2018 oct 2;16(10):365. doi: 10.3390/md16100365.
how to order
mass spectrometry service specification
peptidomics services are performed using nano-flow liquid chromatography and cutting-edge mass spectrometer (q exactive hf and orbitrap fusion lumos).
sample preparation and services
- enrichment of endogenous polypeptides
- 1h for lc-ms/ms analysis
quality standard
- summary includes all methods and data analysis
- reports provided in excel or pdf format, raw files available upon request
turn around time
- typical 4-5 weeks from sample qc acceptance to data report delivery
sample requirements
we accept samples in a variety of formats.
data analysis
data analysis and validation performed with mascot
identification of peptides and proteins
peptide quantification
protein go/cog/pathway annotation
identified peptide matching analysis of specific protein