description
specification
resources
project workflow
references
[1] l. yi, et al., advances in microscale separations towards nanoproteomics applications, 2017.1523: 40–48.
how to order
mass spectrometry service specification
nanoproteomics services are performed using nano-flow liquid chromatography and cutting-edge orbitrap fusion lumos mass spectrometer.
sample preparation and services
- the qc of enzymatic hydrolysis is strictly controlled by lc-ms
- dda-spectral library generated using hplc technology and sample fractionation
- 2h/fraction; 2h for dda analysis
quality standard
- summary includes all methods and data analysis
- reports provided in excel or pdf format, raw files available upon request
turn around time
- typical 5-6 weeks from sample qc acceptance to data report delivery
sample requirements
data analysis
- data analysis and validation performed with mascot
- protein identification and quantification list
- cluster analysis of expression patterns across multiple samples
- protein go/cog/kog/pathway annotation
- go/pathway enrichment analysis of differential proteins
- protein-protein interaction analysis
- protein subcellular localization analysis
- repeatability analysis
- quantitative proteomics and phosphoproteomics correlation analysis